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Batavian
10-18-2014, 10:48 PM
Hello, my name is Joe and I have an 11 year old female black labrador retriever named Chelsea. Chelsea was a rescue. She and her brother Koda were 6 month old puppies found at a “high kill” shelter in Ohio. These type of shelters euthanize after 72 hours. Both her and her brother were saved by some saintly folks, transported to Rochester NY, and put with foster homes until forever homes could be found. I discovered her on Petfinder.com. When I met her for the first time, she came and sat next to me. So she picked me, not vice versa! And I’ve been in love every since. I’m happy to report that both her and her brother Koda (a chocolate male) both found forever homes for these past 10 years or so.

This past summer I noticed that she was panting excessively and drooling. I just figured that she can’t handle the heat very well now that she is older. I recalled some panting back in the summer of 2013 which was a hot summer. But summer 2014 here in western New York was very cool. Of more concern, was her panting and drooling at night.

So I took her in for a checkup and she had a fever! It was 104.8F! And her liver enzymes were elevated. Her liver enzymes had been elevated in the past (more about that later) so the priority was to get the fever down and treat the infection. She was first given Amoxicillin, test after a week, still had fever, so was given some Ciprofloxacin. She was tested again. Fever was gone but still had elevated enzymes, so was given Metronidazole. Tested again and still high liver enzymes. In retrospect, the Metro did nothing for her and was surprised (again in retrospect) that it was prescribed. The fever/infection was probably knocked out by the initial Amoxicillin and/or certainly the Ciprofloxacin. There was been no re-occurance of fever since but her liver enzymes remain high, actually getting worse. Here is a table of her liver enzyme test results.
Normals>> 0-7 23-212 10-100
GGT (U/L) ALKP (U/L) ALT (U/L)
10/16/2012 0 354 133
7/23/2014 13 2000 375
8/9/2014 16 1792 546
8/26/2014 7 1781 250
9/6/2014 12 1736 226
9/16/2014 22 2000 334


Xrays showed no growths or abnormalites other than a slightly enlarged liver. Xrays and ultrasounds showed no bile stones and no detectable bile sludge. Ultrasound and needle biopsy showed normal bladder, spleen, stomach, bowels, gall bladder, kidneys, and adrenal glands. The liver was still slightly enlarged and a little paler than normal. No tumors seen. Slides of the aspirate (needle biopsy) looked like vacuolar degeneration (or vacuolar hepatopathy) which often occurs in Cushing’s or pancreatic issues.

So on to the Cushing’s test. First was the urinalysis. Cortisol = 5.9 ug/dL, creatnine = 17/1 mg/dL. According the urinalysis, it stated hyperadrenocorticism (Cushing’s) was highly unlikely.
A follow-up Cushing’s blood test (dex suppression) was performed. Her cortisol baseline = 3.3 ug/dL, post dex 4hr = 2.0 ug/dL, post dex 8hr = 1.5 ug/dL. I was told that if either the 4 or 8 hour numbers are less than half the baseline, then it’s a positive test for Cushing’s. But the 4 hr is not half and the 8 hr is borderline. Coupled with the urinalysis, the diagnosis was no Cushing’s.

Some side notes. She has some of the symptoms of Cushing’s including drinking more, increased hunger, and panting. But no hair loss, pot belly, obesity. Other info…she’s been tested for heart worm, Lyme, E. canis, and something called A. ph. All negative.

So the diagnosis is ? Right now, she will occasionally pant excessively but no drooling. I having been giving her Denamarin daily which seems to be wonder drug. And she will be starting Science Diet L/D next week. I will do that for a month, then test for the enzymes again.

Sorry for the long drawn out post. It’s probably not Cushing’s or if it is, it's very early in its progression. I will report back the liver enzyme test results in a month. But in the meantime, if any have any ideas about what might be going on, I’d appreciate the comments. Maybe there is a doggie liver forum as great as this Cushing’s forum you could direct me to. If the enzyme results don’t improve, I’m considering taking her to Cornell which is relative close to me and suppose to have an excellent vetrinary school. Right now my local vets are totally perplexed.

labblab
10-19-2014, 05:41 PM
Welcome to you and Chelsea! I'm sorry it has taken us so long to respond to you, but I suspect that may be because we are scratching our heads right alongside you. It sounds as though all the diagnostic testing has proceeded in a very orderly manner, so I am pretty much at a loss as far as suggesting stones that have been left unturned thus far. So in a case as perplexing as Chelsea's, a trip to Cornell may very well be worth taking.

I do want to offer one clarification, however. When interpreting the LDDS test, it is actually the 8-hour value alone that renders the overall diagnosis of Cushing's. If the 8-hour value exceeds the normal range, the result is positive. The normal max is typically 1.4 or 1.5 ug/dl, so on the face of it, Chelsea was likely right at the very border of returning a positive. The "50%" only comes into play if the 8-hour value is positive. If so, then there is a high degree of confidence that the dog suffers from a pituitary rather than an adrenal tumor if either the 4-hour or 8-hour result is less than 50% of the baseline value. If they are not, additional testing is needed to determine the site of the tumor such as ultrasound imaging of the adrenals (which has already been done in your case without seeing any adrenal abnormalities).

Having said all that, there is one more diagnostic blood test for Cushing's that could be performed -- an ACTH stimulation test. We have been told that a small percentage of dogs that test negative on the LDDS will register a positive on the ACTH, especially when the disease is in an early stage. The one thing that makes me doubt that you'll get a positive on the ACTH, though, is the negative urinary cortisol/creatinine test. You would have expected a high reading on that if the ACTH was going to turn out high, too.

These are all reasons why I think you may be well served by a trip to Cornell. I am still wondering whether there may not be a non-Cushing's liver issue at play, but you've already had the ultrasound and biopsy without a definitive answer. So I'd have to defer to the specialists as to the next step.

I am really glad you've joined us, though, and will be very anxious to hear how further diagnostics proceed.

Marianne

Batavian
10-24-2014, 12:56 PM
Thanks for the reply Marianne.

Here is what we are doing. Chelsea just started a new diet today, Science Diet L/D. In addition, she continues to be on one Denamarin a day. We are going to continue this for a month, then have her liver values tested again to see if there has been any improvement. If there is significant improvement, then we will just continue with this for the foreseeable future. If there is no improvement or the values continue to worsen, then we will go to Cornell for further testing and biopsy.

The good news is Chelsea is quite happy. Tail wagging, eager eats food, and other than some excessive panting that occurs at the end of the day, she's doing very well.

Your head scratching has also proved valuable. I've gone to three different clinics and all three also are scratching their collective heads. So if anything, you have confirmed the mystery going on. I am still awaiting for a reply from a fourth clinic but I'm not expecting any new ideas.

The two current theories are...

1) Copper hepatopathy. If this is going on, then the no copper diet should in theory result in improved liver enzyme levels. We will have a better idea in month's time.

2) Cancer. Although all tests have come up negative regarding cancer, there still might be something hidden going on. The only way to tell for sure (so I'm told) would be for a full biopsy. I'm not willing to go that route (full biopsy) yet since all these tests are so fatiguing/stressful to Chelsea. But will cross that bring should the liver diet not result in improvements.

Thank you again for the reply. I read through other peoples threads here and have learned so much. Great forum.

molly muffin
10-24-2014, 08:28 PM
what about a liver bile acid test to see how that is functioning?

Hopefully though the diet and the denamarin will do the trick.

Sharlene and molly muffin